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经口连续给荷Ehrlich腹水癌的BALB/c小鼠克癌7851(AC 7851 160 mg/kg)15d,结果表明癌细胞死亡率明显增加,而腹水量明显减少,二者呈高度相关。AC 7851体外实验用扩散合细胞培养法,结果对空白对照组(不加任何药物)、阴性对照组(黄芪提取液0.6mg/ml)肝癌细胞无杀伤效应;而阳性对照组(阿霉素0.8μg/ml)明显地杀伤了癌细胞(死亡率为14.9±0.8％),AC 7851组(剂量为16μg/ml)也杀伤了大量的癌细胞(死亡率为6.9±1.3％)。扫描电镜下见癌细胞微绒毛大量脱落,许多细胞崩解破坏,破损的细胞粘连在一起形成团块。透射电镜观察到,胞膜、胞核、线粒体及异染色质等均有不同程度的损伤,乃至细胞崩解破坏,核内可见许多圆形的、电子密度高的致密颗粒,其杀伤效应有随AC 7851剂量增加而增加的趋势。在同样条件、同样剂量下,AC 7851对正常淋巴细胞损伤较轻。
Oral continuous feeding of Ehrlich ascites carcinoma BALB/c mice tumors 7851 (AC 7851 160 mg/kg) 15d, the results showed that the cancer cell death rate increased significantly, but the amount of ascites decreased significantly, the two were highly correlated. AC 7851 in vitro proliferation cell culture method, the results of the blank control group (without any drugs), negative control group (Scutellaria extract 0.6mg/ml) liver cancer cells have no killing effect; and positive control group (adriamycin 0.8 Μg/ml) significantly killed the cancer cells (mortality was 14.9 ± 0.8%) and the AC 7851 group (dose 16 μg/ml) also killed a large number of cancer cells (mortality 6.9 ± 1.3%). Scanning electron microscopy revealed that the microvilli of the cancer cells were largely shedding, and many cells collapsed and destroyed, and damaged cells adhered to form clumps. Transmission electron microscopy observed that cell membrane, nucleus, mitochondria, and heterochromatin all had different degrees of damage, and even cell disintegration and destruction. Numerous round, dense particles with high electron density were observed in the nucleus. The killing effect followed. AC 7851 dose increased and increased. In the same conditions, the same dose, AC 7851 less damage to normal lymphocytes.