论文部分内容阅读
目的 :对青年人和老年人的胃癌及癌旁组织进行p53、PCNA、ER、PR表达的对比研究 ,以便明确青年人胃癌和老年人胃癌发生和发展的部分分子机制之间的差异 ,并分析其与胃癌的临床病理指标之间的关系 ,为临床的诊断和治疗提供依据。方法 :用免疫组织化学S P法研究 45例胃癌患者的手术标本 ,其中青年人患者 (≤ 35岁 ) 2 0例 ,老年人患者 (≥ 60岁 ) 2 5例。观察胃癌及癌旁组织的p53、PCNA、ER、PR的表达和临床病理特点 ,并进行分析。结果 :p53和PCNA在青年人和老年人胃癌和癌旁组织中的阳性表达率和表达强度差异无统计学意义。但p53在胃癌组织中的阳性表达率无论是在青年人还是老年人患者中均明显高于癌旁组织 ,差异有显著性。ER、PR在胃癌和癌旁组织中几乎不表达。在 60岁或以上的老年人患者中 ,男性患者占大多数并且胃癌的分化相对较好。 35岁以下的青年人患者中女性患者占多数并且分化程度较差。老年人较青年人胃癌的癌旁组织中有着更多更严重的癌前病变共存。结论 :青年人胃癌和老年人胃癌及癌旁组织有着不同的临床病理特点 ,因此在诊断和治疗上应区别对待。p53的检测有助于判断胃内病变的良恶性质 ,特别是在青年人患者。PCNA可以作为判断胃癌患者预后的较好指标
OBJECTIVE: To compare the expression of p53, PCNA, ER and PR between gastric cancer and paracancerous tissues in young and old, in order to clarify the differences between the molecular mechanisms of gastric cancer and the development of gastric cancer in young people. Its relationship with clinicopathological parameters of gastric cancer provides the basis for clinical diagnosis and treatment. Methods: Immunohistochemical SP method was used to study the surgical specimens of 45 patients with gastric cancer, including 20 young patients (≤ 35 years old) and 25 elderly patients (≥ 60 years old). The expression of p53, PCNA, ER, PR in gastric cancer and adjacent tissues were observed and analyzed. Results: There was no significant difference in the positive expression rate and the expression intensity of p53 and PCNA in gastric cancer and paracancerous tissues between young and old people. However, the positive expression rate of p53 in gastric cancer tissues was significantly higher than that in paracancerous tissues in both young and old patients, the difference was significant. ER, PR in gastric cancer and paracancerous tissues almost no expression. Among elderly patients aged 60 or older, the majority of male patients and gastric cancer are relatively well differentiated. Female patients under 35 years of age are predominantly female and poorly differentiated. Older people have more and more severe precancerous lesions coexisting in their paracancerous tissues than young people. Conclusion: Gastric cancer in young people and gastric cancer in the elderly and their adjacent tissues have different clinicopathological features, so they should be treated differently in diagnosis and treatment. The detection of p53 helps to determine the benign and malignant nature of gastric lesions, especially in young patients. PCNA can be used as a good indicator to judge the prognosis of patients with gastric cancer