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目的网络筛选乳腺癌关键微小RNAs(micro RNAs,miRNAs),并探讨miR-106a-5p对乳腺癌细胞侵袭及迁移能力的影响。方法通过Target Scan和miRanda等工具,构建miRNAs与靶基因以及显著靶向性功能(gene ontology,GO)的调控网络,筛选对乳腺癌有关键调控作用的miRNAs;利用miR-106a-5p agomir及miR-106a-5p antagomir转染MCF-7、T47D乳腺癌细胞株,建立乳腺癌细胞中miR-106a-5p的过表达及敲低体系,Transwell侵袭实验、Transwell迁移实验、划痕实验检测miR-106a-5p对乳腺癌细胞的侵袭、迁移能力的影响。结果在miRNAs-gene-network和miRNAs-GO-network两个网络中调控维度最高的miRNAs基本一致,其中miR-106a-5p是调控维度最高的miRNAs之一;过表达miR-106a-5p显著抑制乳腺癌细胞的侵袭能力和迁移能力(P<0.05),敲低miR-106a-5p则显著增加乳腺癌细胞的侵袭能力和迁移能力(P<0.05)。结论 miR-106a-5p是乳腺癌miRNAs调控网络中的关键miRNAs之一,具有抑制乳腺癌细胞的侵袭及迁移能力的生物学功能。
Objective To screen key microRNAs (miRNAs) in breast cancer and to investigate the effect of miR-106a-5p on invasion and migration of breast cancer cells. Methods Targeted miRNAs and target genes and gene ontology (GO) regulatory networks were constructed by using tools such as Target Scan and miRanda to screen miRNAs that play key regulatory roles in breast cancer. MiR-106a-5p agomir and miR -106a-5p antagomir was transfected into MCF-7 and T47D breast cancer cell lines to establish miR-106a-5p overexpression and knockdown system, Transwell invasion assay, Transwell migration assay and scratch assay to detect miR-106a -5p on invasion and migration of breast cancer cells. Results The most regulated miRNAs in miRNAs-gene-network and miRNAs-GO-network were basically the same, among which miR-106a-5p was one of the most regulated miRNAs. Overexpression of miR-106a-5p significantly inhibited the expression of miR- (P <0.05). Knockdown of miR-106a-5p significantly increased the invasiveness and migration ability of breast cancer cells (P <0.05). Conclusion miR-106a-5p is one of the key miRNAs in miRNAs regulatory networks of breast cancer and has the biological function of inhibiting the invasion and migration of breast cancer cells.