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目的研究胰淀素对大鼠胰岛素和胰高血糖素分泌的影响。方法应用胶原酶消化和不连续密度梯度离心技术建立离体大鼠胰岛模型,研究不同浓度胰淀素对胰岛素和胰高血糖素分泌的影响。结果不同浓度的葡萄糖增加胰岛素的分泌,抑制胰高血糖素的分泌,并都呈线性相关。葡萄糖浓度为0、5.6、11.2mmol/L时,孵育1h,胰淀素(10-10~10-5mol/L)显著抑制胰高血糖素的分泌(P<0.05),且胰高血糖素的分泌与胰淀素浓度呈负相关(P<0.01)。葡萄糖浓度为5.6mmol/L时,不同浓度的胰淀素增加胰岛素分泌,而且两者呈正相关(P<0.05);但葡萄糖浓度为11.2和16.7mmol/L时,10-5mol/L胰淀素对胰岛素的分泌有明显抑制作用(P<0.05)。结论胰淀素对胰高血糖素的分泌有直接抑制作用;在葡萄糖浓度为5.6mmol/L时,胰淀素能增加胰岛素分泌,抑制胰高血糖素分泌;高糖时,高浓度的胰淀素抑制胰岛素分泌。
Objective To study the effect of amylin on the secretion of insulin and glucagon in rats. Methods Rat pancreatic islet model was established by collagenase digestion and discontinuous density gradient centrifugation to study the effect of different concentrations of amylin on insulin and glucagon secretion. Results Different concentrations of glucose increased insulin secretion, inhibition of glucagon secretion, and were linearly related. Glucose concentration of 0,5.6,11.2mmol / L, incubated for 1h, amylin (10-10 ~ 10-5mol / L) significantly inhibited glucagon secretion (P <0.05), and glucagon Secretion and amylin concentration was negatively correlated (P <0.01). Glucose concentration was 5.6mmol / L, different concentrations of amylin increased insulin secretion, and the two were positively correlated (P <0.05); but at glucose concentrations of 11.2 and 16.7mmol / L, 10-5mol / L amylin The secretion of insulin was significantly inhibited (P <0.05). Conclusion Amylin has a direct inhibitory effect on the secretion of glucagon. At glucose concentration of 5.6mmol / L, amylin can increase insulin secretion and inhibit glucagon secretion. In high glucose, high concentration of pancreatic Inhibit insulin secretion.