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通过进一步分析采用全反式维甲酸（ａｌｌ ｔｒａｎｓ ｒｅｔｉｎｏｉｃ ａｃｉｄ， ＡＴＲＡ）诱导人肺腺癌 ＧＬＣ－８２细胞，经ｃＤＮＡ文库消减杂交构建的ｃＤＮＡ消减文库，得到一在ＡＴＲＡ诱导 ＧＬＣ－８２细胞分化过程中激活表达（ｒｅｔｉｎｏｉｃ ａｃｉｄ ｉｎｄｕｃｅｄ， ＲＡＩ）的基因的 ｃＤＮＡ片段，应 用菌落原位杂交技术在胎脑ｃＤＮＡ文库中进行筛选，克隆ＲＡＩ的全长ｃＤＮＡ序列并测 序．ＲＴ－ＰＣＲ分析表明，ＲＡＩ在多种胎儿组织中表达．结果表明，ＲＡＩ可能与细胞分化有 关，并在细胞基本生命活动中发挥重要作用．
By further analysis of all trans retinoic acid (ATRA) induced human lung adenocarcinoma GLC-82 cells by cDNA library subtractive hybridization cDNA subtractive library obtained a ATRA induced GLC-82 cell differentiation process Activation of expression (retinoic acid induced (RAI) cDNA fragments, using colony in situ hybridization in fetal brain cDNA library screening, cloning RAI full-length cDNA sequence and sequencing. RT-PCR analysis showed that RAI was expressed in a variety of fetal tissues. The results showed that RAI may be related to cell differentiation and play an important role in the basic life activities of cells.