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目的探讨DNA损伤修复相关基因XRCC1 Arg399Gln和ERCC2 Lys751Gln多态单独或联合与胃癌发病风险的关系。方法采用病例-对照研究设计,利用聚合酶链反应(PCR)结合限制性片段长度多态性(RFLP)方法,对300例病例和300例对照进行基因型分析,采用多因素logistic回归模型因素分析各基因型以及基因之间交互作用与胃癌发病风险的关系。结果 XRCC1 Arg399Gln多态与胃癌发病风险无关;与携带751 Lys/Lys基因型的个体相比,携带751Gln/Gln基因型的个体胃癌发病的风险增加(OR=2.39,95%CI:1.47~3.89;P<0.001)。XRCC1基因型和ERCC2基因型存在交互作用,携带XRCC1 399Arg/Gln和ERCC2 751Gln/Gln基因型者患胃癌的风险增加(OR=4.63,95%CI:2.11~10.16;P<0.001),携带XRCC1 399Gln/Gln和ERCC2 751 Lys/Gln基因型者患胃癌的风险增加(OR=2.77,95%CI:1.48~5.19;P=0.001)。结论 XRCC1 Arg399Gln和ERCC2 Lys751Gln多态可能是胃癌的遗传易感因素。
Objective To investigate the relationship between DNA damage repair related genes XRCC1 Arg399Gln and ERCC2 Lys751Gln polymorphisms, alone or in combination with the risk of gastric cancer. Methods A case-control study was designed. Genotypes of 300 cases and 300 controls were analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Multivariate logistic regression analysis Relationship between genotype and gene interaction and risk of gastric cancer. Results The polymorphism of XRCC1 Arg399Gln was not associated with the risk of gastric cancer. Compared with individuals carrying 751 Lys / Lys genotype, the risk of developing gastric cancer with 751Gln / Gln genotype increased (OR = 2.39, 95% CI: 1.47-3.89; P <0.001). There was an interaction between genotype XRCC1 and genotype ERCC2. The genotype XRCC1 399Arg / Gln and ERCC2 751Gln / Gln had an increased risk of gastric cancer (OR = 4.63,95% CI: 2.11-10.16; P <0.001) / Gln and ERCC2 751 Lys / Gln genotype had an increased risk of gastric cancer (OR = 2.77, 95% CI: 1.48-5.19; P = 0.001). Conclusion The polymorphisms of XRCC1 Arg399Gln and ERCC2 Lys751Gln may be the genetic predisposing factors for gastric cancer.