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建立了一种同时分离测定血浆中12种磺胺类药物的高效合相色谱(Ultra Performance Convergence Chromatography,UPC2)方法.血浆样品中加入乙腈,涡旋混合后离心,上清液过滤后采用UPC2方法测定,采用Acquity UPC2TM BEH色谱柱,CO2和甲醇作为流动相,梯度洗脱,柱温箱温度40℃,系统背压14.5 MPa.该法在6 min内实现了对12种磺胺类药物的基线分离,定量限为0.5μg/mL,回收率在86.2％～99.5％之间.方法适用于血浆中多种磺胺类药物的同时检测.“,”An analytical method based on UPC 2 has been developed to quantify 12 sulfonamides in plasma .Plasma samples were added with acetonitrile , then the samples were vortexed and centrifuged . The supernatant was filtered and analyzed using UPC 2 .Acquity UPC2TM BEH column was used as the separation column .CO2 and methanol were used as mobile phase , the column oven temperature was 40℃, and the backpressure was 14.5MPa.The method allowed the determination of 12 sulfonamides and metabolites in plsama samples less than 6 min.The limit of quantification was 0.5 μg/mL. Recoveries between 86.2％and 99.5％were obtained .The method is simple , accurate , time-saving and more environmen-friendly , and it is applicable to a variety of sulfonamides detection .