论文部分内容阅读
目的:探讨结肠癌中Hedgehog信号转导通路成员异常活化的情况及其临床意义。方法:选取2009年3月至2010年6月间上海交通大学医学院附属新华医院66例结肠癌组织标本及20例癌旁组织标本,免疫组化检测结肠癌组织中Hedgehog信号转导通路主要蛋白SHH、PTCH1、Gli1、SuFu等的表达,并分析其与结肠癌临床病理特征的关系。结果:在结肠癌组织中SHH和Gli1强阳性表达,而PTCH1、SuFu弱表达(表达率分别为64%、36%、72%及20%);在癌旁组织中,SHH、PTCH1弱表达,而Gli1、SuFu无表达。PTCH1和Gli1的表达与结肠癌的浸润深度有关(P=0.023,P=0.040),而与年龄、性别、病理类型等无关,SHH和SuFu的表达与年龄、性别、病理类型、浸润深度等无关。结论:结肠癌高表达Hedgehog信号转导通路分子,可能参与结肠癌的发生、发展。
Objective: To investigate the abnormal activation of Hedgehog signal transduction pathway in colon cancer and its clinical significance. Methods: From March 2009 to June 2010, 66 cases of colon cancer tissues and 20 cases of adjacent tissues from Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine were selected for immunohistochemistry to detect the major proteins of Hedgehog signaling pathway in colon cancer tissues SHH, PTCH1, Gli1, SuFu and so on, and to analyze its relationship with clinicopathological features of colon cancer. Results: The expression of SHH and Gli1 was strongly positive in colon cancer tissues, while the expression of PTCH1 and SuFu was weak (expression rates were 64%, 36%, 72% and 20%, respectively) The Gli1, SuFu no expression. The expression of PTCH1 and Gli1 was correlated with the depth of invasion of colon cancer (P = 0.023, P = 0.040), but not with age, sex, pathological type, etc. The expression of SHH and SuFu was not related to age, sex, pathological type, . Conclusion: Hedgehog signaling pathway molecules are highly expressed in colon cancer, which may be involved in the occurrence and development of colon cancer.