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Both p21 (WAF1/CIP1) and Gadd45 were activated in a p53-dependent manner in MCF-7 cells after being exposed to ionizing radiation. In order to investigate their roles in DNA damage surveillance, p21~(as)/MCF-7 cells stably transfected by p21 antisense expression plasmid pC-WAF1-AS and Gadd45~(as)/MCF-7 stably transfected by Gadd45 antisense expression plasmid pCMVas45 were established. It was observed that G_1 arrest induced by radiation was significantly reduced in Gadd45~(as)/MCF-7 cells as well as in p21~(as)/MCF-7 cells. Repair of radiation damaged report gene greatly reduced in Gadd45~(as)/MCF-7 and p21~(as)/MCF-7 cells. Apoptosis significantly increased in p21~(as)/MCF-7 after exposure to radiation. These results suggest that both p21 and Gadd45 support cellular survival by taking roles in G_1 arrest and DNA repair, furthermore, p21 protects cells from death by inhibiting apoptosis after exposure to ionizing radiation.
Both order p21 (WAF1 / CIP1) and Gadd45 were activated in a p53-dependent manner in MCF-7 cells after being exposed to ionizing radiation. In order to investigate their roles in DNA damage surveillance, p21 ~ (as) / MCF- It was observed that G_1 arrest induced by radiation was significantly reduced in Gadd45 ~ (as) / stably transfected by p21 antisense expression plasmid pC-WAF1-AS and Gadd45 ~ (as) / MCF-7 stably transfected by Gadd45 antisense expression plasmid pCMVas45 Repair of radiation damaged report gene gene reduced in Gadd45 ~ as / MCF-7 and p21 ~ (as) / MCF-7 cells / MCF-7 cells as well as in p21 asc / These results suggest that both p21 and Gadd45 support cellular survival by taking roles in G_1 arrest and DNA repair, furthermore, p21 protects cells from death by inhibiting apoptosis. Apoptosis was significantly increased in p21 ~ (as) / MCF-7 after exposure to radiation. after exposure to ionizing radiation.