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目的；探讨用酶联免疫吸附法（ＥＬＩＳＡ）测定抗ＥＢ病毒早期抗原ＩｇＧ抗体水平（ＩｇＧ／ＥＡ）在鼻咽癌血清学诊断中的应用。材料和方法：用ＥＬＩＳＡ方法和免疫酶染色方法分别检测５２４例健康人和１２１例鼻咽癌病人血清的ＩｇＧ／ＥＡ水平（ＯＤ）和血清ＩｇＡ抗体对ＥＢ病毒壳抗原（ＩｇＡ／ＶＣＡ）的滴度。结果：以ＯＤ≥０１８作为ＥＬＩＳＡ的阳性值，鼻咽癌诊断的特异性和敏感性分别为９４％（４９３／５２４）和９０％（１０９／１２１），与ＩｇＡ／ＶＣＡ阳性滴度≥１∶８０为标准获得的鼻咽癌诊断特异性９６％（５０３／５２４）和敏感性８９％（１０８／１２１）相一致。以“双阳性”（ＩｇＧ／ＥＡＯＤ≥０１８，ＩｇＡ／ＶＣＡ滴度≥１∶８０）作为鼻咽癌标准，诊断的敏感性为７７％（９３／１２１），特异性１００％（５２４／５２４）。上述结果在１３４例鼻咽癌高危血清样品的测定中得到重复。结论：（１）用ＥＬＩＳＡ测定血清ＩｇＧ／ＥＡ抗体水平也适用于鼻咽癌人群普查。（２）为了提高鼻咽癌血清学诊断的特异性，建议采用“双阳性”（如ＩｇＧ／ＥＡＯＤ≥０１８，ＩｇＡ／ＶＣＡ滴度≥１∶８０）作为鼻咽癌人群普查中的血清学阳性标准。
Objective To investigate the application of ELISA to determine the level of anti-Epstein-Barr virus IgG (IgG / EA) in the serological diagnosis of nasopharyngeal carcinoma. MATERIALS AND METHODS: Serum IgG / EA levels (OD) and serum IgA antibodies against Epstein-Barr virus capsid antigen (IgA / VCA) in 524 healthy subjects and 121 nasopharyngeal carcinoma patients were detected by ELISA and immunoenzyme staining respectively. degree. Results: The specificity and sensitivity of OD ≥ 0.18 for ELISA were 94% (493/524) and 90% (109/121), respectively. The positive rate of IgA / VCA titer ≥ 1:80 is the standard 96% (503/524) diagnostic specificity for NPC and 89% (108/121) for sensitivity. The diagnostic sensitivity was 77% (93/121) and 100% (524/100) with “double positive” (IgG / EAOD≥0.18, IgA / VCA titer≥1:80) 524). The above results were repeated in the determination of 134 high-risk serum samples of NPC. Conclusion: (1) Serum IgG / EA antibody levels by ELISA are also suitable for the screening of NPC patients. (2) In order to improve the specificity of serological diagnosis of nasopharyngeal carcinoma, it is recommended to use “double positive” (such as IgG / EAOD≥0.18, IgA / VCA titer≥1:80) Learn positive standards.