论文部分内容阅读
采用免疫胶体金标记电镜技术对水稻(Oryza sativa subsp. indica cv. 浙农952)叶片中的Rubisco及其活化酶(RCA)进行细胞器定位和定量, 同时用免疫扩散法进行叶片含量分析, 研究了这两种酶含量及活力的日变化。结果表明Rubisco主要分布于叶绿体,RCA分布于叶绿体和线粒体中;光合速率(Pn)、Rubisco 初始活力和RCA活力与光合日变化密切相关;在光照最强的13时, 出现光合“午休”, 叶绿体中Rubisco的密度有一定程度降低,而全叶的总Rubisco保持稳定, Rubisco初始活力也有明显的“午休”, 这意味着体内Rubisco的活力除受RCA调节外, 可能还与叶绿体中Rubisco的分布有关。RCA活力变化与叶绿体中RCA含量变化较为一致, 表明RCA在叶绿体中的分布对调节其本身活力和Rubisco活性有重要作用。
Rubisco and its activated enzyme (RCA) in the leaves of Oryza sativa subsp. Indica cv. Zhenong 952 were localized and quantitated by immunogold labeling electron microscopy and electron microscopy. The leaf contents were analyzed by immunodiffusion method. The diurnal changes of both enzyme content and vitality. The results showed that Rubisco mainly distributed in the chloroplast and RCA distributed in the chloroplast and mitochondria. The photosynthetic rate (Pn), Rubisco initial activity and RCA activity were closely related to the photosynthetic diurnal variation. At the 13th illumination, photosynthesis “nap” The Rubisco density decreased to a certain extent, while the total Rubisco in the whole leaf remained stable. The initial activity of Rubisco also showed a significant “lunch break”, which implied that the activity of Rubisco in vivo may be related to the distribution of Rubisco in the chloroplast . The change of RCA activity in chloroplast was consistent with that of RCA, indicating that the distribution of RCA in chloroplast plays an important role in regulating the activity of Rubisco and its activity.