论文部分内容阅读
目的建立野葛花中葛花苷、尼泊尔鸢尾异黄酮、鸢尾黄素-7-O-木糖葡萄糖苷、鹰嘴豆芽素A4种异黄酮成分含量测定方法。方法采用HPLC法。色谱柱为Kromasil C18柱(200 mm×4.6 mm,5μm),以体积分数为1%的乙酸水溶液-甲醇-乙腈为流动相,线性梯度洗脱,流速为0.8 mL.min-1,检测波长为254 nm,柱温35℃。结果葛花苷、尼泊尔鸢尾异黄酮、鸢尾黄素-7-O-木糖葡萄糖苷、鹰嘴豆芽素A质量浓度分别在42.8~856 mg.L-1、9.12~182.4 mg.L-1、13.2~264 mg.L-1、4.20~840 mg.L-1内呈良好线性关系,相关系数分别为0.999 9、0.999 9、0.999 1、0.999 3,平均回收率分别为100.7%(RSD=2.2%,n=9)、99.1%(RSD=2.4%,n=9)、98.6%(RSD=1.6%,n=9)和98.3%(RSD=1.5%,n=9)。结论该方法简便、准确、重现性好,可用于野葛花的质量控制。不同花期和不同药用部位的野葛花中4种指标成分的含量差异较大。
OBJECTIVE To establish a method for the determination of isoflavones content of kudzu glycosides in Pueraria lobata, Neispermum isatidis, tectorigenin-7-O-xyloglucoside and chickpea sp. Methods HPLC method. The column was a Kromasil C18 column (200 mm × 4.6 mm, 5 μm) with a linear gradient of 0.8 mL · min-1 with a 1% acetic acid aqueous solution-methanol-acetonitrile as the mobile phase. The detection wavelength was 254 nm, column temperature 35 ℃. Results The results showed that the mass concentration of kaempferol, Nepalsia isoflavone, tectorigenin-7-O-xyloglucoside and chickpea sp. A were 42.8-856 mg.L-1, 1.12-182.4 mg.L-1, 13.2-264 mg.L-1,4.20-840 mg.L-1, the correlation coefficients were 0.999 9,0.999 9,0.999 1 and 0.999 3, respectively. The average recoveries were 100.7% (RSD = 2.2 %, n = 9), 99.1% (RSD = 2.4%, n = 9), 98.6% (RSD = 1.6%, n = 9) and 98.3% (RSD = 1.5%, n = 9). Conclusion The method is simple, accurate, reproducible and can be used for the quality control of Pueraria lobata. There were significant differences among the four index components in different flowering and medicinal parts of Pueraria lobata.