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由交链格孢菌橘致病型(Alternaria alternata tangerine pathotype)引起的柑橘链格孢褐斑病(Alternaria brown spot,ABS)是柑橘上的主要病害,目前主要采用杀菌剂防治。本研究以采自中国各柑橘链格孢褐斑病发生区的54个交链格孢菌橘致病型菌株为供试群体,采用刃天青微平板法测定了病原菌孢子萌发和菌丝生长对戊唑醇和苯醚甲环唑的敏感性。结果表明:刃天青微平板法最佳体系中的培养基为完全培养基(CM),刃天青终浓度为40μmol/L,孢子浓度为105个/m L。戊唑醇和苯醚甲环唑的有效抑制中浓度(EC50)范围分别为0.304~0.885μg/m L和0.367~0.873μg/m L,平均值分别为0.562和0.609μg/m L。正态分布检测结果显示:这2种药剂抑制交链格孢菌橘致病型群体的EC50值均符合正态分布,因此认为所得EC50值可作为病原菌对戊唑醇和苯醚甲环唑的敏感性基线。本研究结果可为田间防治褐斑病和监测交链格孢菌对戊唑醇和苯醚甲环唑的抗药性奠定基础。
Citrus Alternaria brown spot (Alternaria brown spot) caused by Alternaria alternata tangerine pathotype is one of the major diseases in citrus. At present, antibacterial agents are used to control it. In this study, 54 strains of Alternaria alternata isolates from all citrus loci in China were selected as test groups. The spore germination and mycelial growth Sensitivity to tebuconazole and difenoconazole. The results showed that the optimum medium for resazurin microplate culture was medium (CM), the final concentration of resazurin was 40 μmol / L, and the concentration of spores was 105 / m L. The effective inhibitory concentrations (EC50) for tebuconazole and difenoconazole ranged from 0.304 to 0.885 μg / mL and from 0.367 to 0.873 μg / mL, with mean values of 0.562 and 0.609 μg / mL, respectively. The results of normal distribution test showed that the EC50 values of these two agents in inhibiting the genotype of Alternaria alternata were all in normal distribution. Therefore, the obtained EC50 value could be regarded as the sensitivity of the pathogen to tebuconazole and difenoconazole Sexual baseline. The results of this study may lay the foundation for the prevention and treatment of brown spot in the field and monitoring the resistance of Alternaria alternata to tebuconazole and difenoconazole.