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研究了氯化血红素（Ｈｅｍｉｎ）模拟辣根过氧化物酶（ＨＲＰ），对羟基苯乙酸（ＰＨＰＡＡ）作 底物，高效液相色谱（ＨＰＬＣ）柱后衍生荧光法测定Ｈ２Ｏ２和水溶性有机过氧化物的方法。采用 Ｈｅｍｉｎ作催化剂柱后衍生反应的最佳ｐＨ值约为１１，与荧光检测ｐＨ值一致，使得以往ＨＰＬＣ 柱后衍生方法所需的高压泵从３台减少到２台，而一些不能作为ＨＲＰ底物的羟烷基过氧化 物在ｐＨ值≥１０的溶液中迅速水解为Ｈ２Ｏ２从而得到测定。优化了测定Ｈ２Ｏ２和甲基过氧化 氢（ＣＨ３ ＯＯＨ， ＭＨＰ）的条件。最佳条件下 Ｈｅｍｉｎ方法测定 Ｈ２Ｏ２的检测限为 ９．０ × １０－９ ｍｏｌ／Ｌ， 测定 ＭＨＰ的检测限为 ２．０ × １０－７ ｍｏｌ／Ｌ。
The effects of hydrogen peroxide (HRP), p-hydroxyphenylacetic acid (PHPAA) as substrates and high performance liquid chromatography (HPLC) on the determination of H2O2 and water-soluble organic acids Oxide method. The optimum pH of the derivatization reaction with Hemin as catalyst is about 11, which is consistent with the fluorescence detection of the pH value. This reduces the number of high-pressure pumps required for conventional post-HPLC derivatization from 3 to 2, and some can not be used as HRP The hydroxyalkyl peroxide of the substance is rapidly hydrolyzed to H202 in a solution of pH 10 or higher for determination. Optimization of the determination of H2O2 and methyl hydrogen peroxide (CH3 OOH, MHP) conditions. Under the optimal conditions, the detection limit of Hemin method for H2O2 was 9.0 × 10-9 mol / L, and the detection limit of MHP was 2.0 × 10-7 mol / L.