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由于缺少有效的方法,通常难以测定水产养殖池中微生物的总生产率。本研究以RNA和DNA的含成率对水体中微生物(藻类和细菌)的总生产作定量分析,同时对混养、单养、投饵或施肥各种条件下的养殖池作自养性生产过程中原初(藻类)生产率的定量测定。根据DNA合成率所得的总生产率为218～6620微克碳/升·小时,其时异养性生产率35～3518微克碳/升·小时。以吸收~(14)C的碳酸氢盐得出的原初生产率4～336微克碳/升·小时。异养性生产率在8次观测资料中有7次占微生物总生产率的50％以上。RNA,DNA的合成率,微生物总生产率与三磷酸腺苷(ATP)生物量密切相关(P<0.01),而自养性生产率则与叶绿素—α或三磷酸腺苷生物量无显著相关。在此项研究中用DNA合成法测定微生物总生产率较之以往用常规方法估测的总生产率要高得多。
Due to the lack of efficient methods, it is often difficult to measure the overall productivity of microorganisms in aquaculture ponds. In this study, the total production of microorganisms (algae and bacteria) in water was quantitatively analyzed with the inclusion rates of RNA and DNA, and the autotrophic production process was carried out on the culture pond under various conditions of polyculture, monoculture, feeding or fertilization Central Plains primary (algae) production rate of quantitative determination. The total yield based on the DNA synthesis rate is 218 to 6620 micrograms of carbon per liter hour, at which time the heterotrophic productivity is 35 to 3518 micrograms of carbon per liter hour. The initial yield from absorption of ~ (14) C bicarbonate was 4 to 336 micrograms of carbon per liter hour. Heterotrophic productivity 7 times in 8 observations accounted for more than 50% of the total microbial productivity. The synthesis rates of RNA and DNA, and the total microbial productivity were closely related to the ATP content (P <0.01), while the autotrophic productivity did not correlate with chlorophyll-α or ATP content. The total microbial productivity measured by DNA synthesis in this study is much higher than the overall productivity estimated by conventional methods.