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目的探讨采用Hep G2细胞株建立裸鼠人肝癌原位模型的方法,评价其生物学特性。方法取人肝癌细胞株Hep G2制备皮下移植瘤,然后将瘤块植入裸鼠肝左叶,建立原位移植模型,观察荷瘤鼠生存状态。裸鼠全身衰竭时处死、取瘤,HE染色及免疫组化法观察瘤组织的病理特点、血管生长及肿瘤细胞凋亡情况。结果种瘤成功率90%,荷瘤鼠中位生存期76.7d,肿瘤直径(16.8±2.2)mm,体积(965.3±333.3)mm~3,重量(1.1±0.4)g。瘤组织早期具有旺盛的增殖能力,晚期癌组织中Caspase3、TUNEL呈强阳性表达。结论由原位移植法建立的裸鼠人肝癌模型,造模时间短、成瘤率高,生物学行为与临床肝癌相似,是基因药物筛选研究的理想模型。
Objective To investigate the method of establishing the in situ model of human hepatocellular carcinoma in nude mice by using Hep G2 cell line to evaluate its biological characteristics. Methods Human hepatoma cell line Hep G2 was used to prepare subcutaneously transplanted tumor. Then the tumor mass was implanted into the left lobe of the liver of the nude mouse to establish an orthotopic transplantation model. The survival status of the tumor-bearing mice was observed. The nude mice were sacrificed in the process of systemic failure. The pathological features, angiogenesis and tumor cell apoptosis of the tumor tissues were observed by HE staining and immunohistochemistry. Results The tumor success rate was 90%. The median survival time was 76.7 days in tumor - bearing mice. The diameter of tumor was 16.8 ± 2.2 mm, the volume was (965.3 ± 333.3) mm ~ 3 and the weight was (1.1 ± 0.4) g. Early tumor tissue with strong proliferative capacity, advanced cancer tissues Caspase3, TUNEL showed a strong positive expression. Conclusion The model of human hepatocellular carcinoma in nude mice established by orthotopic transplantation has the advantages of short modeling time, high rate of tumorigenesis and similar biological behavior with clinical liver cancer. It is an ideal model for screening of gene and drug.