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目的研究过敏性休克死亡豚鼠心、气管、喉头白细胞介素10(Interleukin-10,IL-10)表达的死后变化规律,寻找过敏性休克死亡的法医学诊断的客观指标。方法参照文献建立豚鼠过敏性休克死亡动物模型,采用免疫组织化学的方法,应用图像分析技术,检测各组豚鼠心、气管、喉头IL-10在死后0h、48h(4℃冷藏)、5d(-20℃冷冻)、7d(-20℃冷冻)四个时间点的表达量。结果在健康的豚鼠(对照组)心肌细胞、气管、喉头粘膜上皮细胞中IL-10均呈弱阳性表达,而在过敏性休克死亡豚鼠(实验组)心肌细胞、气管、喉头粘膜上皮细胞中IL-10均呈较强阳性表达,各实验组与相应的对照组之间均有显著差异(p<0.05);随着死后时间的延长,在死后冷藏48 h或冷冻7 d内,IL-10在心、气管、喉头的表达量呈缓慢减少趋势,但各实验组之间IL-10的表达量均无显著差异(p>0.05)。结论死后冷藏48h或冷冻7d内尸体,其心肌细胞、气管、喉头粘膜上皮细胞IL-10呈较强阳性表达,这可能成为过敏性休克死亡法医学鉴定的较好的客观指标。
Objective To study the changes of the expression of Interleukin-10 (IL-10) in the heart, trachea and larynx of guinea pigs who died of anaphylactic shock and find out the objective indexes of forensic diagnosis for the death of anaphylactic shock. Methods The animal model of guinea pig anaphylactic shock was established with reference to the literature. The expression of IL-10 in heart, trachea and larynx of guinea pigs was detected by immunohistochemical method and image analysis technique at 0h, 48h (4 ℃), 5d -20 ℃ frozen), 7d (-20 ℃ frozen) expression of the four time points. Results IL-10 was weakly positive in cardiomyocytes, trachea and laryngeal mucosal epithelium in healthy guinea pigs (control group), while in IL-10 in guinea pigs (experimental group) -10 were significantly positive expression of each experimental group and the corresponding control group were significantly different (p <0.05); with the extension of post-mortem time, after 48 hours of post-mortem or frozen within 7 days of freezing, IL -10 in the heart, trachea and larynx showed a slowly decreasing trend, but there was no significant difference in IL-10 expression between the experimental groups (p> 0.05). Conclusion The positive expression of IL-10 in myocardial cells, trachea and larynx mucosa epithelial cells after 48h or 7d cryopreservation can be a better objective index for forensic identification of anaphylactic shock.