论文部分内容阅读
目的:建立紫外分光光度法测定自制对氨基水杨酸钠肠溶片的含量,并与药典方法相比较。方法:紫外分光光度法为以pH7．4的磷酸盐缓冲液(PBS)为溶剂,以298 nm为检测波长,药典方法为永停滴定法。以t-检验法对两种测定方法的结果进行分析。结果:对氨基水杨酸钠在pH 7．4的PBS中的线性范围为4．0～20．1μg·ml-1,平均回收率为100．6％,RSD为0．7％(n=5),其测定结果与永停滴定法无显著性差异(P>0．05)。结论:采用紫外分光光度法测定对氨基水杨酸钠包衣片的含量,比永停滴定法更为简单、快速。
OBJECTIVE: To establish a UV spectrophotometric method for the determination of the content of self-made p-aminosalicylic sodium enteric-coated tablets, and to compare with the pharmacopoeia method. Methods: UV spectrophotometry was pH7.4 phosphate buffer (PBS) as solvent, with 298 nm as the detection wavelength, Pharmacopoeia method for the stop-stop titration. The results of the two assays were analyzed by t-test. RESULTS: The linear range of sodium p-aminosalicylate in PBS at pH 7.4 was 4.0-20.1 μg · ml-1 with an average recovery of 100.6% and a RSD of 0.7% (n = 5). There was no significant difference between the results and the elution titration (P> 0.05). Conclusion: UV-spectrophotometry determination of sodium aminosalicylate coated tablets content, than Yong stop titration method is more simple and fast.