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溶液法合成了二(去甲基斑蝥酸根)合钴(Ⅱ)酸二(2-氨基吡啶鎓)配合物(Hapy)2[Co(DCA)2].6H2O(DCA=去甲基斑蝥酸根,C8H8O5;Hapy=2-氨基吡啶鎓,C5H7N2)。通过元素分析、摩尔电导、红外光谱、热重分析和X-射线单晶衍射对配合物进行了结构表征。该配合物为三斜晶系P空间群,中心离子配位数为6。利用荧光光谱法和粘度法对配合物与DNA之间的相互作用进行了研究。结果表明,配合物以部分插入模式与DNA键合,猝灭常数Ksq为0.18。同时,利用荧光光谱研究了配合物与牛血清白蛋白(BSA)的相互作用。结果显示,配合物能与BSA发生较强的键合作用,键合常数Ka=3.88×106L.mol-1。体外抗增值活性结果显示,配合物对人肝癌细胞(SMMC-7721)和人胃癌细胞(MGC80-3)的抑制活性均强于去甲基斑蝥酸钠。
(DCA = desmethylcatecholate) was synthesized by the solution method. The complex of bis (2-aminopyridinium) (Hapy) 2 [Co C8H8O5; Hapy = 2-aminopyridinium, C5H7N2). The complex was characterized by elemental analysis, molar conductance, IR, TG and X-ray single crystal diffraction. The complex is triclinic P space group, the central ion coordination number of 6. The interaction between the complex and DNA was studied by fluorescence spectroscopy and viscometry. The results showed that the quenching constant Ksq was 0.18 when the complex was partially inserted into DNA. At the same time, the interaction between the complex and bovine serum albumin (BSA) was studied by fluorescence spectroscopy. The results show that the complex with BSA stronger bonding, bonding constant Ka = 3.88 × 106L.mol-1. The anti-proliferation activities in vitro showed that the inhibitory activities of the complexes on human hepatocellular carcinoma cells (SMMC-7721) and human gastric cancer cells (MGC80-3) were stronger than sodium demethyl cantharidate.