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目的：分子克隆编码牛心线粒体特长脂酰辅酶Ａ脱氢酶的ｃＤＮＡ基因．方法：基因的分离是采用抗体筛选技术并采用已知的氨基末端和肽链内部的氨基酸序列等多种方法核对其可靠性．结果：所获ｃＤＮＡ片段对应于ＲＮＡ印迹法所示的约２４ｋｂ的ｍＲＮＡ片段，可翻译为６５５个氨基酸分子序列（７０５ｋＤａ）．比较脂酰辅酶Ａ脱氢酶家族的肽链序列显示主要不同在于它们的信号肽与其它脂酰辅酶Ａ脱氢酶比较，其催化部位高度保守．在羧基末端，存在一个和“亮氨基拉锁”特征序列类似的结构（亮５６８到亮５８９），为该蛋白四级结构的形成提供了一个潜在机制．结论：我们分离的ｃＤＮＡ克隆编码牛心线粒体内膜特长脂酰辅酶Ａ脱氢酶的同功酶．
OBJECTIVE: To molecular clone the cDNA encoding the gene encoding fatty acid coenzyme A dehydrogenase in mitochondria. Methods: The gene isolation was based on the antibody screening technique and the amino acid sequence of the amino terminus and peptide chain were used to check the reliability of the method. Results: The obtained cDNA fragment corresponded to about 2.4 kb mRNA fragment shown by Northern blotting, and could be translated into 655 amino acid sequence (70.5 kDa). The main difference compared to the peptide sequences of the fatty acyl-CoA dehydrogenase family is that their signal peptides are highly conserved in their catalytic sites compared to other fatty acyl-CoA dehydrogenases. At the carboxy terminus, there is a similar structure to the “leucoaractone” signature sequence (bright 568 to bright 589), providing a potential mechanism for the formation of the quaternary structure of this protein. Conclusions: The cDNA clone we isolated encodes the isozymes of bovine cardiac mitochondrial membrane-specific fatty acyl-CoA dehydrogenase.