论文部分内容阅读
目的分析2012年清远市流感病原学监测结果,了解流感病毒的流行特征以及流行优势毒株,为流感防控工作提供科学依据。方法对清远市监测哨点医院就诊的流感样病例鼻咽拭子用RT-PCR法检测流感病毒核酸,同时用狗肾传代细胞(MDCK细胞)法分离流感病毒并进行毒株鉴定。结果 2012年共检测流感样病例鼻咽拭子标本489份,RT-PCR检出阳性115份,阳性率为23.52%,其中季节性H3型、B型和A未分型阳性率分别为45.22%、43.48%、11.30%。细胞培养分离出毒株97株,阳性率为19.84%,其中季节性H3N2型、Bv型、By型分离率分别为61.86%、35.05%和3.09%。1~2月份以B型流感病毒为主要优势毒株,3~7月份流感流行的优势毒株以季节性H3N2型为主,同时也伴随B型流感病毒流行,而8~12月份均未检测到流感阳性病例。结论 2012年清远市流感流行优势毒株为季节性H3N2型和B型,流行季节主要在春夏季。RT-PCR检测流感病毒的特异性和敏感性均较细胞培养法高,但细胞培养法作为流感病原学监测的基础,仍然是实验室诊断的金标准。
Objective To analyze the surveillance results of influenza etiology in Qingyuan City in 2012 to understand the epidemiological characteristics of influenza virus and the predominant strains of influenza and provide a scientific basis for prevention and control of influenza. Methods The influenza virus nucleic acid was detected by RT-PCR in flu-like cases treated by sentinel hospital in Qingyuan City. Influenza virus was isolated by MDCK cell sorting and identified. Results In 2012, 489 nasopharyngeal swab samples were detected in influenza-like cases, and 115 were positive by RT-PCR, the positive rate was 23.52%. The positive rates of seasonal H3, B and A non-typing were 45.22% , 43.48%, 11.30%. 97 strains were isolated from the cell culture, the positive rate was 19.84%. The seasonal H3N2 type, Bv type and By type separation rates were 61.86%, 35.05% and 3.09% respectively. From January to February, influenza B virus was the predominant strain. The predominant influenza strains from March to July were predominantly seasonal H3N2 and influenza B, but not detected from August to December To flu-positive cases. Conclusion In 2012, the prevalence strains of influenza in Qingyuan City were seasonal H3N2 and B, and the epidemic season was mainly in spring and summer. The specificity and sensitivity of RT-PCR in the detection of influenza virus are higher than those in cell culture, but the cell culture method is still the gold standard for laboratory diagnosis as the basis of influenza etiology.