论文部分内容阅读
目的：研究鼻咽癌上皮细胞系CNE－2Z及其克隆株CNE－2Z－H5的枝型特征和标记染色体。方法：常规细胞培养和制片后进行染色体G显带，按人类细胞遗传学国际命名体制（ISCN）进行核型分析．结果：CNE－2Z的染色体武目在74～96之间．众数为86～87；CNE－2Z－H5的染色体数目在83～93之间，众数为86～88。两种细胞均有一系列结构复杂的标记染色体，其中18条出现在大部被分析的细胞中，其结构变化主要涉及3号、6号、9号和15号染色体。结论：鼻咽癌的核型具有高度异质性，不同细胞系之间的核型特征差别明显。
AIM: To study the characteristics of branching and marker chromosomes of nasopharyngeal epithelial cell line CNE-2Z and its clone CNE-2Z-H5. Methods: Conventional cell culture and post-production chromosome G banding, according to the International Cytogenetics of Human Cytology (ISCN) for karyotyping. Results: The chromosome of CNE-2Z was between 74 and 96. The number of 86 to 87; CNE-2Z-H5 chromosome number between 83 to 93, the mode of 86 to 88. Both cells have a series of complex marker chromosomes, of which 18 appear in most of the cells analyzed. The structural changes mainly involve chromosomes 3, 6, 9 and 15. Conclusion: The karyotypes of NPC are highly heterogeneous, and the differences of karyotype characteristics between different cell lines are obvious.