取生药粉水煎醇沉,浓缩成膏,以甲醇提取,氧化镁吸附,于索氏提取器中甲醇提取,乙醚沉淀物为总皂甙。薄层层离皂甙斑点,刮入离心管中,加0.5%H_2[P(MO_2O_7)_6]冰乙酸液4ml,浓硫酸2ml,3000r/min离心30分钟,于波长495nm处测定吸收度标准曲线。以样品吸收度查出计算皂甙含量。 The raw powders were decocted with alcohol, concentrated into a paste, extracted with methanol, and adsorbed with magnesium oxide. The extract was extracted with methanol from a Soxhlet extractor. The ether precipitate was total saponin. The thin lamellar saponin spot was scraped into a centrifuge tube, and 0.5 ml of H2[P(MO_2O_7)_6] glacial acetic acid solution was added in 4 ml, concentrated sulfuric acid (2 ml), centrifuged at 3000 r/min for 30 minutes, and the absorbance standard curve was measured at a wavelength of 495 nm. The saponin content was determined from the sample absorbance.