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为了阐明不同抗性香蕉种质对枯萎病4号小种(Foc4)侵染的反应机制,本研究从香蕉中克隆到两个与病程相关的基因,类甜蛋白基因(Ma TLP)和β-1,3-葡聚糖酶基因(Ma BGLU)。对枯萎病菌侵染后Ma TLP和Ma BGLU转录水平进行实时荧光定理PCR实验分析,结果显示:Ma TLP和Ma BGLU基因的表达水平在病原菌侵染后随着侵染时间的增加而迅速增加,尤其在病原菌侵染后51 h,Ma TLP在抗性种质FHIA-25和GCTCV-119中的绝对表达量远远高于在感病种质巴西蕉中的表达量,表明其在香蕉与病原菌互作中发挥重要的作用;而在抗性种质Rose中,Ma TLP和Ma BGLU的表达水平远低于在感病种质巴西蕉中的表达量,并且机械损伤与病原菌处理间,基因的表达量相差不大,说明其存在不同的抗性机制。本研究结果表明不同抗性的香蕉种质可能具有不同的抗性机制,可为进行香蕉与枯萎病特异互作机制的研究奠定了基础。
In order to elucidate the mechanism of different resistant banana germplasms to Foc4 infection, two disease-related genes, Ma TLP and β- 1, 3-glucanase gene (Ma BGLU). Real-time fluorescence PCR analysis of Ma TLP and Ma BGLU transcript levels after Fusarium oxysporum infection showed that the expression levels of Ma TLP and Ma BGLU genes increased rapidly with the increase of infection time after infection by pathogenic bacteria, especially At 51 h after pathogen infection, the absolute expression level of Ma TLP in resistant FHIA-25 and GCTCV-119 was much higher than that in susceptible germplasm Banana, indicating that the interaction between banana and pathogen The expression level of Ma TLP and Ma BGLU in resistant germplasm Rose was much lower than that in susceptible germplasm M. brasiliensis, and the gene expression between mechanical injury and pathogen treatment The amount of difference is not large, indicating its existence of different resistance mechanisms. The results of this study showed that different resistant banana germplasm may have different resistance mechanisms, which laid the foundation for the research on the mechanism of the specific interaction between banana and blight.