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作者以改良的 Gamborg’s B5固体介质为培养基对东北红豆杉 Taxus cuspidata 愈伤组织进行了培养,并对培养物中的紫杉烷类进行了分析。以该植物嫩枝诱导愈伤组织。诱导和培养条件:改良的 Gamborg’s B5培养基含维生素、蔗糖与0.5 mg/L NAA(在此生长的培养物为 CR-5)或与1.0 mg/L NAA 和0.3 mg/
The authors cultured taxus cuspidata Taxus cuspidata using a modified Gamborg’s B5 solid medium and analyzed the taxanes in the culture. The callus was induced by the shoots of the plant. Induction and culture conditions: Modified Gamborg’s B5 medium with vitamins, sucrose, and 0.5 mg/L NAA (cultured here for CR-5) or with 1.0 mg/L NAA and 0.3 mg/