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目的筛查膀胱浸润性尿路上皮癌差异表达的基因,并探讨MTFSS1基因对膀胱癌T24细胞侵袭能力的影响。方法应用基因芯片结合生物信息学软件筛查膀胱浸润性尿路上皮癌差异表达的基因,并通过荧光定量PCR和Western Blot进行验证;通过转染技术,应用MTT和Transwell小室法分别检测MTSS1基因对膀胱癌T24细胞增殖和侵袭能力的影响。结果膀胱浸润性尿路上皮癌和其癌旁组织表达差异2倍以上的基因有1392个,荧光定量PCR和Westem Blot显示MTSS1在癌组织中较在癌旁组织中表达降低,两者差异35倍,P=0.0065,主要在趋化因子信号转导通路中发挥重要作用。转染结果显示MTSS1基因高表达列T24细胞增殖能力没有影响,而能明显抑制T24细胞的侵袭转移能力(P<0.05)。结论 MTSS1基因在膀胱浸润性尿路上皮癌中表达下调是其促进该肿瘤侵袭转移的重要因素之一。
Objective To screen differentially expressed genes in bladder invasive urothelial carcinoma and to investigate the effect of MTFSS1 gene on invasiveness of bladder cancer T24 cells. Methods Genes differentially expressed in bladder invasive urothelial carcinoma were screened by gene chip combined with bioinformatics software and verified by real-time PCR and Western Blot. MTSS1 gene pairs were detected by MTT assay and Transwell chamber assay Effect of Bladder Cancer T24 Cell Proliferation and Invasion. Results There were 1392 genes with more than 2-fold difference in bladder invasive epithelial carcinoma and paracancerous tissues. Fluorescent quantitative PCR and Westem Blot showed that the expression of MTSS1 in cancer tissues was lower than that in paracancerous tissues, with a difference of 35-fold , P = 0.0065, which play an important role in chemokine signal transduction pathway. Transfection results showed that MTSS1 gene highly expressed T24 cell proliferation had no effect, but could significantly inhibit T24 cell invasion and metastasis (P <0.05). Conclusions The down-regulation of MTSS1 gene in bladder invasive urothelial carcinoma is one of the important factors that promote its invasion and metastasis.