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目的:观察连翘对豚鼠离体肠管运动的影响,以期探讨其止呕作用的机制。方法:以呕吐相关受体激动剂为工具药,利用离体恒温浴槽,观察连翘对豚鼠离体回肠收缩的影响。结果:连翘能够抑制离体肠管的自发活动,表现为收缩张力降低,并呈剂量依赖性关系。乙酰胆碱(acetylcholine,Ach)、组织胺(histamine,His)、五羟色胺(5-hydroxytryptamine,5-HT)能够兴奋肠管,使张力升高、振幅变大,连翘高(10 g·L-1)、中(5 g·L-1)、低(2 g·L-1)剂量均能抑制以上3种工具药所致肠管收缩,降低其收缩张力和振幅,但对频率无明显影响。多巴胺(dopamine,DA)能够抑制肠管收缩,表现为张力降低、振幅变小,连翘高剂量和中剂量能够拮抗DA的肠管松弛作用,使张力升高、振幅变大;连翘低剂量能够进一步使张力降低,但使振幅增大。结论:连翘可以抑制豚鼠回肠运动,其机制可能是通过阻断肠平滑肌上的M受体、H1受体、5-HT受体和D2受体,也可能是对肠管的直接抑制作用,其止呕机制尚待进一步深入研究。
Objective: To observe the effects of forsythia on intestinal motility in guinea pigs in order to explore its mechanism of vomiting. Methods: Vomiting-related receptor agonist was used as a tool drug, and the effects of forsythia on the ileum contraction in isolated guinea pigs were observed by using an ex-vivo thermostatic bath. RESULTS: Forsythia suspensa inhibited the spontaneous activity of isolated intestine, showing a decrease in contractile tension and in a dose-dependent manner. Acetylcholine (Ach), histamine (His) and 5-hydroxytryptamine (5-HT) were able to excite the intestines and increase the tension, amplitude and forsythia (10 g · L -1) The doses of 5 g · L-1 and 2 g · L-1 all inhibited the intestinal contractions induced by the above three kinds of tools and decreased the contractile tension and amplitude, but had no significant effect on the frequency. Dopamine (DA) can inhibit the contraction of the intestine, showing a decrease in tension, amplitude smaller, Forsythia high dose and dose can antagonize the damp intestinal relaxation, so that tension increased, the amplitude increased; Forsythia lower dose can be further The tension is reduced, but the amplitude is increased. CONCLUSION: Forsythia suspensa can inhibit the ileum movement in guinea pigs, and its mechanism may be that it can directly inhibit the intestine by blocking the M receptor, H1 receptor, 5-HT receptor and D2 receptor on the intestinal smooth muscle. Vomit mechanism remains to be further studied.