目的　观察化疗对NK细胞活性的损伤程度。方法　收集 1999～ 2 0 0 1年 32例上皮性卵巢癌 ,观察化疗前后NK细胞活性的变化。然后与IL 2共培养 ,观察其活性恢复情况。结果　化疗后与化疗前比较 ,NK细胞活性下降明显 ,差异有统计学意义。应用IL 2共培养后 ,化疗前NK细胞活性明显升高 ;化疗后第 1～ 3疗程细胞活性升高有显著性 ,第 4～ 6疗程活性无显著性变化。结论　化疗损伤了NK细胞的活性 ,早期损伤程度较轻 ,能恢复 ;晚期损伤程度较重 ,短期内不能恢复 Objective To observe the extent of chemotherapy-induced NK cell injury. Methods 32 cases of epithelial ovarian cancer from 1999 to 2001 were collected to observe the changes of NK cell activity before and after chemotherapy. Then co-cultured with IL 2 to observe the recovery of their activity. Results Compared with before chemotherapy, NK cell activity decreased significantly after chemotherapy, the difference was statistically significant. After co-culture with IL 2, the activity of NK cells increased significantly after chemotherapy; the activity of cells in the first to the third courses of chemotherapy increased significantly after chemotherapy, while the activity of the 4th to 6th courses did not change significantly. Conclusions Chemotherapy can damage the activity of NK cells, with a lesser degree of early injury and recovery; the degree of advanced injury is severe, and can not be recovered in a short period of time