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目的:观察递增负荷运动后大鼠血清雌二醇、胫骨雌激素受体和转化生长因子-β1的表达,探讨递增负荷运动后骨组织的适应机制。方法:30只SD雌性大鼠随机分为对照组、一次性大强度运动组、长期大强度运动组。ELISA法测定血清E2含量,免疫组化测定ER和TGF-β1的表达,RT-PCR法测定ER mRNA和TGF-β1mRNA表达水平。结果:一次性大强台运动后血E2,ER mRNA和TGF-β1mRNA水平均无显著性差异。长期大强度运动后血E2显著下降(P<0.01),ER mRNA和TGF-β1mRNA均显著下降(P<0.05)。一次性运动组胫骨ER、TGF-β1的积分光密度和平均光密度组间比较均无显著性差异。长期大强度运动组ER、TGF-β1的积分光密度显著下降(P<0.05),平均光密度显著下降(P<0.01)。结论:长期大强度跑台运动可降低大鼠血清E2,下调胫骨ER和TGF-β1表达。
OBJECTIVE: To observe the expression of estrogen, tibia estrogen receptor and transforming growth factor-β1 in rat after progressive exercise, and to explore the adaptive mechanism of bone tissue after progressive exercise. Methods: Thirty SD female rats were randomly divided into control group, one-time intensive exercise group and long-term intensive exercise group. Serum E2 levels were measured by ELISA. The expression of ER and TGF-β1 was detected by immunohistochemistry. The mRNA and protein levels of ER and TGF-β1 were determined by RT-PCR. Results: There was no significant difference in E2, ER mRNA and TGF-β1 mRNA levels after one-time exercise. After long-term high-intensity exercise, serum E2 decreased significantly (P <0.01), ER mRNA and TGF-β1 mRNA decreased significantly (P <0.05). One-time exercise group tibial ER, TGF-β1 integral optical density and average optical density between the groups were no significant difference. The integral optical density of ER and TGF-β1 decreased significantly (P <0.05) and the average optical density decreased significantly in long-term intensive exercise group (P <0.01). Conclusion: Long-term high-intensity treadmill exercise can reduce serum E2 and down-regulate the expression of ER and TGF-β1 in tibia.