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目的:观察大鼠原位种植肝癌模型中的新生血管的生长情况,研究肿瘤血管生长因子基因的表达和肝癌新生血管之间的关系。 方法:采用Walker256癌肉瘤接种40只Wistar大鼠制作原位种植肝癌模型,用微血管计数以及免疫组织化学染色法检测平滑肌激动蛋白(α-SMA)的表达来标志肿瘤新生血管。血管肉皮细胞生长因子基因的检测采用原位杂交法进行。 结果:Walker256癌肉瘤接种后1、2、3 wk肿瘤微血管密度分别为(15±4.3)/HP、(17±3.6)/HP、(45±7.8)/Hp。大血管及正常微血管(毛细血管除外)周围α-SMA呈强阳性染色。在肝癌组织中可见到一些形态不规则的微血管呈弱阳性或阴性染色是肿瘤诱导的新生血管,肝癌组织有大量新生血管形成,新生血管的数量与微血管密度呈正相关,Walker256癌肉瘤接种后3 wk较前2wk有显著性增加。正常肝脏组织仅有少量VEGF mRNA表达,肝癌组织接种后1wk即有多种细胞表达VEGF。新生血管较密集的肿瘤组织VEGF的表达较多。VEGF的表达与微血管密度和肿瘤组织中新生血管的数量呈正相关。 结论:原位接种肝癌中有大量新生血管形成,平滑肌激动蛋白(α-SMA)的表达可以用来标记肿瘤新生血管。肝癌组织中血管内皮细胞生长因子的表达增加可能和肿瘤新生血管的形成有一定的关系。
OBJECTIVE: To observe the growth of neovascularization in rat hepatocarcinoma model in situ and to study the relationship between the expression of tumor vascular growth factor gene and neovascularization of liver cancer. Methods: Forty Wistar rats were inoculated with Walker256 carcinosarcoma to establish a model of orthotopic liver cancer. Microvascular count and immunohistochemical staining were used to detect the expression of α-SMA to mark tumor neovascularization. Vascular endothelial growth factor (VEGF) gene was detected by in situ hybridization. Results: The microvessel density of Walker256 carcinosarcoma was (15 ± 4.3) / HP, (17 ± 3.6) / HP and (45 ± 7.8) / Hp respectively at 1 and 3 weeks after inoculation. Α-SMA around the large blood vessels and normal capillaries (except capillaries) showed strong positive staining. In HCC there are some irregular microvascular micro-positive or negative staining is tumor-induced neovascularization, a large number of neovascularization of liver cancer tissue, the number of neovascularization and microvessel density was positively correlated, Walker256 carcinosarcoma 3wk Compared with the previous 2wk significant increase. Only a small amount of normal liver tissue VEGF mRNA expression, liver cancer tissue 1wk after inoculation of a variety of cells that express VEGF. More neovascular tumor tissue VEGF expression more. The expression of VEGF was positively correlated with the microvessel density and the number of neovascularization in tumor tissue. Conclusion: There is a large number of neovascularization in in situ hepatocellular carcinoma. The expression of smooth muscle actin (α-SMA) can be used to mark tumor neovascularization. The increased expression of vascular endothelial growth factor in hepatocellular carcinoma may be related to the formation of neovascularization.