用1,6-二苯基-1,3,5-己三烯(DPH)作荧光探剂嵌入正常小鼠和糖尿病小鼠完整淋巴细胞膜脂区,用荧光偏振法测定膜脂区流动性。结果表明糖尿病小鼠淋巴细胞膜的微粘度(η)明显高于正常小鼠。将糖尿病小鼠淋巴细胞置于正常小鼠血清中,37℃温育30min后,其膜的η可回复至正常小鼠水平。提示糖尿病小鼠淋巴细胞膜的η与细胞膜胆固醇含量有关。 1,6-diphenyl-1,3,5-hexatriene (DPH) was used as a fluorescent probe embedded in the membrane lipids of intact lymphocytes of normal mice and diabetic mice. Membrane lipid zone fluidity was measured by fluorescence polarization method. The results showed that the micro-viscosity (η) of lymphocytes in diabetic mice was significantly higher than that of normal mice. Diabetic mouse lymphocytes were placed in normal mouse serum. After incubation at 37°C for 30 min, the membrane’s η could return to normal mice. It is suggested that the η of the lymphocyte membrane of diabetic mice is related to the content of cell membrane cholesterol.