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[目的]检测人肝癌细胞株FOXP3基因启动子区域的甲基化状态。[方法]选取人肝癌细胞株HepG2、SMMC7721为研究对象,采用亚硫酸氢盐修饰后的测序方法检测FOXP3启动子区CpG岛的甲基化水平。[结果]在人肝癌细胞株HepG2与SMMC7721中,FOXP3基因启动子区域CpG岛甲基化水平均比较高,其中SMMC7721的甲基化频率约80%;HepG2的甲基化频率约86%。[结论]人肝癌细胞株SMMC7721与HepG2中FOXP3基因启动子处于高甲基化状态,为进一步的肝癌细胞FOXP3基因的表观遗传学研究打下一定基础。
[Objective] To detect the methylation status of FOXP3 promoter region in human hepatoma cell line. [Methods] Human hepatocellular carcinoma cell lines HepG2 and SMMC7721 were selected as research objects. The methylation level of CpG island in FOXP3 promoter region was detected by bisulfite modified sequencing. [Results] The methylation levels of CpG island in FOXP3 promoter region were higher in human hepatocellular carcinoma cell lines HepG2 and SMMC7721. The methylation frequency of SMMC7721 was about 80% and the methylation frequency of HepG2 was 86%. [Conclusion] The promoter of FOXP3 gene in human hepatocellular carcinoma cell lines SMMC7721 and HepG2 is hypermethylated, which lays a foundation for the further study on the epigenetic study of FOXP3 gene in hepatocellular carcinoma cells.