论文部分内容阅读
目的研究山楂原花青素(HPC)和维生素C(VC)联合应用对胰岛素抵抗(IR)大鼠肾脏损伤的保护作用。方法采用高脂膳食法诱导IR大鼠,造模后继续高脂喂养2周,制备IR肾病大鼠模型,并检测空腹血糖、血清胰岛素以及尿液各项指标、微量白蛋白(mAlb)水平;将造模后的50只IR肾病大鼠分为模型组、HPC(56 mg/kg)组、VC(180 mg/kg)组、HPC(56 mg/kg)+VC(180 mg/kg)组和罗格列酮(2 mg/kg)组,另取10只正常大鼠作为对照组,各组均ig给药,持续20周,测定大鼠空腹血糖、胰岛素、尿糖水平,检测尿液中肌酐(Cr)、尿素氮(UN)、尿酸(UA)、总蛋白水平以及mAlb、N-乙酰-β-D-葡萄糖苷酶(NAG)24 h排泄量,光镜下观察肾脏组织病理学改变。结果与对照组比较,模型组大鼠空腹血糖、血清胰岛素以及尿糖水平显著升高(P<0.01),尿中UN、UA、Cr水平显著升高(P<0.01),尿总蛋白、mAlb水平以及尿NAG排泄量均显著升高(P<0.01)。而HPC和VC联合应用后以上指标均显著下降(P<0.01),较HPC组和VC组作用显著(P<0.01),与罗格列酮组效果相近。HPC+VC组大鼠肾脏组织显微结构有明显改善。结论 HPC与VC联合应用可改善IR大鼠肾脏功能,对肾脏有一定的保护作用。
Objective To study the protective effects of combined application of hawthorn proanthocyanidins (HPC) and vitamin C (VC) on renal injury induced by insulin resistance in rats. Methods IR rats were induced by high - fat diet. The model rats with IR nephropathy were induced by continuous high fat diet for 2 weeks. The levels of fasting blood glucose, serum insulin, urine and microalbumin (mAb) were measured. Fifty IR nephrotic rats were divided into model group, HPC group (56 mg / kg), VC group (180 mg / kg), HPC group And rosiglitazone (2 mg / kg), and the other 10 normal rats were taken as control group. Each group was given ig for 20 weeks. The fasting blood glucose, insulin and urine glucose level were measured and urine The creatinine (Cr), urea nitrogen (UU), uric acid (UA), total protein and 24 h excretion of mAlb and N-acetyl-β-D-glucosidase (NAG) change. Results Compared with the control group, the level of fasting blood glucose, serum insulin and urine glucose in model group were significantly increased (P <0.01), and the levels of urine UN, UA and Cr were significantly increased (P <0.01) Levels and urinary NAG excretion were significantly increased (P <0.01). However, the combination of HPC and VC reduced the above indexes significantly (P <0.01), and had a significant effect compared with HPC group and VC group (P <0.01), similar to rosiglitazone group. The microstructure of kidney in HPC + VC group was significantly improved. Conclusions HPC combined with VC can improve kidney function of IR rats and protect the kidneys.