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目的观察酶促牛黄(CBCG)对小鼠的抗惊厥、抗炎作用,初步评价CBCG的药用价值。方法 (1)抗惊厥实验:分2个子实验,各取50只小鼠随机分为生理盐水组(NS组)、戊巴比妥钠组(PBS组)及CBCG高、中、低剂量组各10只,分别采用尼可刹米和最大电休克法制备小鼠惊厥模型,观察CBCG的抗惊厥作用。(2)抗炎实验:分2个子实验,各取60只小鼠随机分为NS组、阿司匹林组(ASP组)、天然牛黄组(NCB组)及CBCG高、中、低剂量组各10只,分别采用二甲苯致小鼠耳廓肿胀、角叉菜胶致小鼠足肿胀的炎性反应模型,观察CBCG的抗炎作用。结果 (1)与NS组比较,PBS组、CBCG各剂量组均能延长尼可刹米对小鼠的惊厥潜伏期,提高最大电休克小鼠的惊厥阈值,减少最大电惊厥发作的小鼠例数,差异有统计学意义(P<0.05,P<0.01),且PBS组作用优于CBCG组(P<0.01),而CBCG不同浓度组间差异无统计学意义(P>0.05)。(2)与NS组比较,ASP组、NCB组及CBCG高、中剂量组均可抑制二甲苯致小鼠耳廓肿胀度(P<0.05,P<0.01),且以CBCG高剂量组作用最强(P<0.05,P<0.01)。与NS组比较,ASP组、NCB组及CBCG各剂量组药后3～5 h均可抑制小鼠后足趾肿胀度(P<0.05,P<0.01),且以NCB组和CBCG高剂量组作用最强(P<0.05,P<0.01)。结论 CBCG对小鼠具有抗惊厥、抗炎作用。
Objective To observe the anticonvulsant and anti-inflammatory effects of enzymatic bezoar (CBCG) on mice and to evaluate the medicinal value of CBCG. Methods (1) Anticonvulsant experiment: Divided into 2 sub-experiments, 50 mice in each group were randomly divided into saline group (NS group), pentobarbital sodium group (PBS group) and CBCG high, medium and low dose groups 10 mice were used to produce mice convulsions model with Nicorandil and maximum electroshock method respectively to observe the anticonvulsant effect of CBCG. (2) Anti-inflammatory experiment: divided into two sub-experiments, each of 60 mice were randomly divided into NS group, aspirin group (ASP group), natural bezoar group (NCB group) and CBCG high, medium and low dose groups of 10 , Respectively, using xylene-induced mouse ear swelling, carrageenan induced by foot swelling in mice inflammatory response model to observe the anti-inflammatory effect of CBCG. Results (1) Compared with NS group, PBS group and CBCG groups could prolong the latent period of ecotatic convulsion in mice, raise the threshold of seizure in maximal electroshock mice and reduce the number of mice with maximal electroconvulsive seizure (P <0.05, P <0.01), and the effect of PBS group was better than that of CBCG group (P <0.01). There was no significant difference between CBCG groups (P> 0.05). (2) Compared with NS group, the auricle swelling degree of mice induced by xylene could be inhibited by ASP and NCB group and CBCG high and medium dose group (P <0.05, P <0.01) Strong (P <0.05, P <0.01). Compared with the NS group, ASP group, NCB group and CBCG group could inhibit the degree of swelling of the rear toe of the mice 3 ~ 5 h after treatment (P <0.05, P <0.01), and the NCB group and CBCG high dose group The strongest (P <0.05, P <0.01). Conclusion CBCG has anticonvulsant and anti-inflammatory effects on mice.