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在体外分别以P.gingivalis LPS和E.coli LPS刺激HAECs,实时荧光定量PCR技术检测ICAM-1基因表达,蛋白质印迹技术检测ICAM-1膜蛋白表达。采用SPSS18.0软件包中ANOVA对数据进行分析。结果:P.gingivalis LPS作用6h后开始诱导ICAM-1 mRNA表达,14h达到高峰,22h仍有高表达,各时间点与未刺激组相比,差异均有统计学意义(P<0.05);而E.coli LPS作用2h后即开始诱导ICAM-1 mRNA表达,14h达到高峰,22h仍有高表达,各时间点与未刺激组相比差异均有统计学意义(P<0.05)。两种LPS均
HAECs were stimulated with P. gingivalis LPS and E. coli LPS respectively in vitro, ICAM-1 gene expression was detected by real-time fluorescence quantitative PCR and protein expression of ICAM-1 was detected by Western blotting. Data was analyzed using ANOVA in the SPSS18.0 software package. Results: After 6h, the expression of ICAM-1 mRNA was induced by P. gingivalis LPS, and reached the peak at 14h and remained high at 22h. The differences were statistically significant (P <0.05) at all time points compared with those in unstimulated group After 2h, the expression of ICAM-1 mRNA began to be induced by E.coli LPS, reached its peak at 14h, and remained high at 22h. There were significant differences between the two groups at each time point (P <0.05). Both LPS are