论文部分内容阅读
人类肝炎可由甲型肝炎病毒(HAV)乙型肝炎病毒、非甲非乙型肝炎病毒和“δ”病毒所致。甲型肝炎病情较缓和,不致慢性肝炎;但HAV感染仍为人类肝炎发病、偶而死亡的重要原因。鉴于HAV细胞培养、分子克隆及血清学技术的迅速进展,目前对HAV与宿主的相互关系、疫苗及流行病学特点等有一定知识,并致力于HAV疫苗制备的研究。 一、HAV的基本特性 HAV为裸露的小RNA病毒,呈球形,直径约27μm,20面体对称。沉降系数为16S,氯化銫浮力密度为1.325g/cm~3。由VP_1～VP_4四种多肽构成蛋白质的衣壳。衣壳内为单股RNA(32～35S),约含7500个核苷酸,其3′端为多聚腺苷酸。病毒体RNA本身具感性。最近Najanian已报道
Human hepatitis can be caused by Hepatitis A virus (HAV) Hepatitis B virus, non-A, non-B hepatitis virus and “delta” virus. Hepatitis A is more moderate and does not cause chronic hepatitis; however, HAV infection is still an important cause of human hepatitis and death occasionally. Given the rapid progress of HAV cell culture, molecular cloning and serological techniques, there is currently some knowledge of HAV-host interactions, vaccines and epidemiological features, and is committed to the study of HAV vaccine preparation. First, the basic characteristics of HAV HAV naked picornavirus, was spherical, about 27μm in diameter, 20-sided symmetry. Sedimentation coefficient is 16S, cesium chloride buoyant density of 1.325g / cm ~ 3. The VP_1 ~ VP_4 four polypeptides constitute the capsid of the protein. Single-stranded RNA within the capsid (32-35S), containing about 7500 nucleotides, the 3 ’polyA. The virion RNA itself has sensitivities. Recently Najanian has reported