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建立了抑制电导离子色谱梯度洗脱的方法,将结构相似的α-,β-甘油磷酸钠进行有效分离并分析各自含量。色谱条件:Ionpac AS11-HC阴离子色谱柱和Ionpac AG11-HC保护柱,NaOH梯度洗脱,抑制电导检测器。α-,β-甘油磷酸钠在4.98～49.8 mg/L范围内呈良好线性,线性方程分别为Y=0.0225X-0.011和Y=0.0247X-0.0188,线性相关系数均为0.9999。α-甘油磷酸钠保留时间和峰面积的相对标准偏差分别为2.7%和0.73%,加标回收率为100.4%～107.9%。β-甘油磷酸钠保留时间和峰面积的相对标准偏差分别为2.2%和0.29%,加标回收率为100.8%～105.9%。α-,β-甘油磷酸钠的检出限(以S/N=3计)都是0.26 mg/L。方法可用于实际样品检测。
A method of inhibiting the gradient elution of ion-exchange chromatography was established, and the structure-like α, β-sodium glycerophosphate was effectively separated and analyzed. Chromatographic conditions: Ionpac AS11-HC anion column and Ionpac AG11-HC guard column, NaOH gradient elution, inhibition of conductance detector. The linearities of α- and β-glycerophosphates in the range of 4.98-49.8 mg / L were Y = 0.0225X-0.011 and Y = 0.0247X-0.0188, respectively. The linear correlation coefficients were all 0.9999. The relative standard deviations (RSDs) of retention time and peak area of α-glycerophosphate were 2.7% and 0.73%, respectively. The recoveries were 100.4% -107.9%. The relative standard deviations (RSDs) of retention time and peak area of β-glycerophosphate were 2.2% and 0.29%, respectively. The recoveries were 100.8% -105.9%. The detection limit of α- and β-glycerophosphate (both S / N = 3) was 0.26 mg / L. The method can be used for actual sample testing.