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本实验观察了胰岛素样生长因子Ⅱ（ＩＧＦ－Ⅱ）对大鼠离体培养黄体细胞孕酮生成的影响，并对其作用机制进行了探讨。结果显示，ＩＧＦ－Ⅱ能显著地促进大鼠离体培养黄体细胞孕酮生成并呈剂量－效应关系，同时还能促进３Ｈ－亮氨酸（３Ｈ－ｌｅｎ）掺入黄体细胞蛋白质的合成，促进３Ｈ－胸腺嘧啶（３Ｈ－ＴｄＲ）掺入ＤＮＡ的合成，而上述效应分别被放线菌酮（ＣＹＸ）和放线菌素Ｄ（ＡｃｔＤ）所抑制。此外，ＩＧＦ－Ⅱ对大鼠高体黄体细胞内源性ｃＡＭＰ和ｈＣＧ诱导的ｃＡＭＰ含量均无影响，这提示ＩＧＦ－Ⅱ对大鼠黄体细胞孕酮生成的促进作用与其增强黄体细胞蛋白质与ＤＮＡ的合成有关，而其跨膜信息传递似不通过ｃＡＭＰ系统。
In this study, the effect of insulin-like growth factor Ⅱ (IGF-Ⅱ) on progesterone production in cultured rat luteal cells was investigated and its mechanism of action was also discussed. The results showed that IGF-Ⅱ could significantly promote the progesterone production in rat luteal cells in vitro and in a dose-effect relationship, and at the same time promote the synthesis of 3H-len protein into luteal cells, 3H-thymine (3H-TdR) incorporation into DNA, whereas the above effects are inhibited by cycloheximide (CYX) and actinomycin D (ActD), respectively. In addition, IGF-II had no effect on endogenous cAMP and hCG-induced cAMP levels in rat corpus luteum cells, suggesting that IGF-II may promote progesterone production in rat luteal cells and enhance the protein and DNA of luteal cells Synthesis, and its transmembrane information delivery does not seem to pass cAMP system.