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目的 观察免疫应答时胸腺内降钙素基因相关肽(CGRP)能神经纤维的可塑性 ,为CGRP在胸腺的调节作用提供线索。方法 用PPD免疫Balb/c小鼠 ,胸腺切片用免疫组织化学法处理 ,光镜下观察。结果 在正常Balb/c小鼠胸腺被囊、小梁内有较丰富的CGRP能神经纤维分布 ,胸腺实质中也有阳性纤维存在。用PPD免疫 2 8d后 ,胸腺被囊、小梁内血管周围的CGRP阳性纤维明显增加 ,这些纤维直径较粗、膨体少 ,多数围绕血管形成网状 ;有的聚集成束直行。胸腺实质内阳性纤维的数量少 ,免疫后较对照组略有增加 ,分布不均。此外 ,胸腺髓质中的一些较大的、三角形或椭圆形的上皮样细胞 ,亦呈CGRP免疫反应阳性。结论 CGRP与胸腺在免疫应答时的结构、机能变化有关
Objective To observe the plasticity of calcitonin gene related peptide (CGRP) nerve fibers in the thymus during the immune response and provide clues for the regulation of CGRP in the thymus. Methods Balb / c mice were immunized with PPD. Thymus sections were treated with immunohistochemistry and observed under light microscope. Results In the normal Balb / c mouse thymus capsule, the trabeculae are more abundant distribution of CGRP nerve fibers, there are also positive fibers in the thymus parenchyma. After immunized with PPD for 2 to 8 days, the thymus capsule and CGRP-positive fibers around the blood vessels in the trabeculae were significantly increased. These fibers were thicker with less bulge and mostly formed reticulation around the blood vessels. Some gathered in bundles straight. Thymus parenchyma within the small number of positive fibers, slightly increased after immunization compared with the control group, uneven distribution. In addition, some large, triangular or oval epithelial-like cells in the thymus medulla are also immunoreactive with CGRP. Conclusions CGRP is related to the structure and function of thymus in immune response