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目的:制备亚硒酸锰()-叶绿酸钠,并测定其对小鼠的急性毒性及对小鼠肝癌H22的抑制情况。方法:以蚕沙提取叶绿素,由叶绿素制备脱镁叶绿酸,与锰络合后制得锰()叶绿酸,再以亚硒酸化合制得亚硒酸锰()-叶绿酸钠;测定亚硒酸锰()-叶绿酸钠的急性毒性及对小鼠肝癌H22的抑制情况。结果:作者首次合成了亚硒酸锰()-叶绿酸钠。测得其LD50=1175mg·kg-1(95%的可信区间为851～1621mg·kg-1;其对小鼠肝癌H22的抑癌率为45～47%(灌胃剂量为500mg·kg-1)。结论:亚硒酸锰()-叶绿酸钠常温下性质稳定,毒性小,对小鼠肝癌H22有明显的抑制作用,可进一步做临床试验
OBJECTIVE: To prepare manganese selenite () - sodium chlorophyllin and determine its acute toxicity to mice and its inhibitory effect on H22 in mice. Methods: Chlorophyll was extracted from silkworm excrement, pheophorbide was prepared from chlorophyll, manganese () chlorophyll was prepared after complexation with manganese, and then manganese selenite ; Determination of manganese selenite () - sodium chlorophyllin acute toxicity and inhibition of mouse liver cancer H22. Results: The authors first synthesized manganese selenite () - sodium chlorophyllin. Its LD50 = 1175mg · kg-1 (95% confidence interval 851 ~ 1621mg · kg-1); its tumor suppressor rate of mouse hepatoma H22 was 45 ~ 47% (intragastric dose of 500mg · kg- 1) .Conclusion: Manganese selenite () - sodium chlorophyllin has stable property and low toxicity at room temperature, and has obvious inhibitory effect on mouse hepatocellular carcinoma H22 and can be further used in clinical trials