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用ELISA法测定24例结、直肠癌患者血浆、外周血单个核细胞（PBMC）、癌组织TNF水平，与良性病变、正常人对照。结果：（1）结、直肠癌组血浆TNF水平显著升高（P＜0．001），DukesD期患者血浆TNF水平显著高于DukesB或C期（P<0．001）；PBMC经LPS诱生的TNF水平与对照组比较天显著性差异（P＞0．05）；（2）结、直肠癌组织TNF水平显著高于正常（P<0.01），肿瘤最大直径在5．0cmLi上者，癌组织TNF水平显著升高（P＜0．05）；（3）肿瘤根治术后血浆TNF水平显著下降（P＜0．01），而肿瘤非根治术者血浆TNF水平手术前后天显著差异（P＞0.05）；PBMC上清液TNF水平在根治与非根治术前后均无明显差异（P＞0．05）．结果提示：肿瘤负荷是结、直肠癌患者内源性TNF升高的主要原因；内源性TNF水平升高与结直肠癌生长、转移有关。
The levels of TNF in plasma, peripheral blood mononuclear cells (PBMC), and cancer tissues in 24 patients with colorectal cancer were determined by ELISA and compared with benign lesions and normal controls. Results: (1) Plasma TNF levels were significantly higher in the colorectal and rectal cancer groups (P<0.001). Plasma TNF levels were significantly higher in Dukes D patients than in Dukes B or C (P<0.001); PBMCs were induced by LPS. The TNF level was significantly different from the control group (P>0.05); (2) The TNF levels in the colorectal cancer tissue were significantly higher than normal (P<0.01). The maximum tumor diameter was 5.0 cmLi. Tissue TNF levels were significantly elevated (P<0.05); (3) Plasma TNF levels were significantly decreased after radical surgery (P<0.01), whereas plasma TNF levels in non- radical operations were significantly different before and after surgery (P<0.05). > 0.05); PBMC supernatant TNF levels in the radical and non-radical surgery were no significant difference (P> 0.05). The results suggest that tumor burden is the main cause of the increase of endogenous TNF in patients with colorectal cancer; the increase of endogenous TNF levels is related to the growth and metastasis of colorectal cancer.