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目的建立枝穗大黄中大黄素含量测定方法的一种方法。方法采用双波长薄层扫描法,测定波长λ_S=290nm,参比波长λ_R=342nm。结果枝穗大黄中大黄素的平均含量为0.62%,平均回收率为97.5%。结论用双波长薄层扫描法测定,方法简单、灵敏。
Objective To establish a method for the determination of emodin in Radix et Rhizoma Rhei. Methods The dual-wavelength thin-layer scanning method was used to determine the wavelength λ_S = 290nm and the reference wavelength λR = 342nm. Results The average content of emodin in the rhizome of rhizome was 0.62% and the average recovery was 97.5%. Conclusion Dual-wavelength thin-layer scanning method, the method is simple and sensitive.