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A new facile fluorescence probing strategy, which was based on N-doped carbon dots(NCDs) and methyl parathion hydrolase(MPH), was developed for the determination of parathion-methyl(PM). The fluorescence intensity of NCDs-MPH system was proportional to PM concentration in the range of 2.38–73.78 mmol/L, with a detection limit of 0.338 mmol/L. Moreover, the present simple and facile method could be used to determine methyl parathion in environmental and agricultural samples successfully.Furthermore, the detection mechanism of this system is inner filter effect and molecular interactions between NCDs and p-nitrophenol, which is the hydrolysis product of PM catalyzed by methyl parathion hydrolase.
A new facile fluorescence probing strategy, which was based on N-doped carbon dots (NCDs) and methyl parathion hydrolase (MPH), was developed for the determination of parathion-methyl (PM). The fluorescence intensity of NCDs-MPH system was proportional to PM concentration in the range of 2.38-73.78 mmol / L, with a detection limit of 0.338 mmol / L. Furthermore, the present simple and facile method could be used to determine methyl parathion in environmental and agricultural samples successfully. mechanism of this system is inner filter effect and molecular interactions between NCDs and p-nitrophenol, which is the hydrolysis product of PM catalyzed by methyl parathion hydrolase.