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本文应用Ames试验,CHL细胞体外染色体畸变试验检测了脱氧胆酸和石胆酸的遗传毒性;还观察了脱氧胆酸和石胆酸对NIH/3T3细胞的恶性转化作用。并应用划痕标记染料示踪技术(SLDT)观察了胆汁酸对细胞间隙连接通讯(GJIC)的抑制作用。结果表明,脱氧胆酸和石胆酸无诱发基因突变和染色体畸变作用,也无诱发培养细胞的恶性转化作用。因此本实验未证明脱氧胆酸和石胆酸是遗传毒性致癌物。然而,脱氧胆酸和石胆酸对NIH/3T3细胞GJIC均有明显抑制作用,说明胆汁酸在大肠癌的发生中是一种促癌剂
In this paper, Ames test, CHL cell chromosome aberration test in vitro detection of genotoxicity of deoxycholic acid and lithocholic acid; Also observed deoxycholic acid and lithocholic acid on NIH / 3T3 cells malignant transformation. The inhibitory effect of bile acid on GJIC was observed by using scratch-labeled dye-tracing technique (SLDT). The results showed that deoxycholic acid and lithocholic acid did not induce gene mutation and chromosomal aberration, nor induced the malignant transformation of cultured cells. Therefore, this experiment did not prove that deoxycholic acid and lithocholic acid are genotoxic carcinogens. However, deoxycholic acid and lithocholic acid have a significant inhibitory effect on GJIC of NIH / 3T3 cells, indicating that bile acid is a kind of cancer promoter in the development of colorectal cancer