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应用平行平板流动腔装置研究毛细血管内皮细胞内皮素（ＥＴ）代谢与剪切作用时间的相关关系。将培养的人胚肾小球血管单层内皮细胞置于剪应力分别为０、５×１０－５、１×１０－４和１．５×１０－４Ｎ／ｃｍ２的定常层流中剪切作用２５小时，样品中的ＥＴ分泌量用放射免疫法测定。结果表明，剪切作用时间与内皮细胞ＥＴ的代谢活动有密切相关，ＥＴ分泌量高低不仅取决于剪切应力大小，而且还取决于剪切作用时间长短。ＥＴ分泌量随着剪切作用时间的增减而非线性地增减，在０Ｎ／ｃｍ２、５×１０－５Ｎ／ｃｍ２、１×１０－４Ｎ／ｃｍ２、１．５×１０－４Ｎ／ｃｍ２时ＥＴ累积分泌量最快速最关键的时段分别是流动剪切后７．８－１７．７ｈ、５．３－１６．１ｈ、６．１－１５．６ｈ、８．１－２２．３ｈ；产生最大分泌率时刻相应发生在流动剪切后１２．８ｈ、１０．７ｈ、１０．９ｈ、１５．２ｈ，相应的最大分泌率分别为４５．４１ｐｇ／ｃｍ２．ｈ、４９．１ｐｇ／ｃｍ２．ｈ、７２．５ｐｇ／ｃｍ２．ｈ、３３．５ｐｇ／ｃｍ２．ｈＥＴ累积分泌量和分泌速率随剪切作用时间变化的关系可用Ｌｏｇｉｓｔｉｃ方程来描述。获得了反映ＥＴ分泌规律的大量特征方程和特征数，为了解体内发生于血管壁的病理生?
The application of parallel plate flow chamber device to study the relationship between capillary endothelial cell endothelin (ET) metabolism and shear time. Cultured human embryonic glomerular monolayer endothelial cells were sheared in a steady laminar flow of shear stress of 0, 5 x 10-5, 1 x 10-4 and 1.5 x 10-4 N / cm2, respectively Twenty-five hours, the amount of ET secreted in the sample was measured by radioimmunoassay. The results show that the time of shearing is closely related to the metabolic activity of ET in endothelial cells. The level of ET secretion depends not only on the magnitude of shear stress but also on the duration of shearing. The amount of ET secretion increased or decreased nonlinearly with the increase or decrease of the shearing time, and at 0 N / cm2, 5 × 10-5 N / cm2, 1 × 10-4 N / cm2 and 1.5 × 10-4 N / cm2 ET cumulative secretion of the most critical period of time is the flow of shear 7.8-17.7h, 5.3-16.1h, 6.1-15.6h, 8.1-22.3h; produce the largest The corresponding secretion rates occurred at 12.8h, 10.7h, 10.9h and 15.2h after flow-shear, respectively, with the corresponding maximum rates of 45.41pg / cm2. h, 49.1 pg / cm2. h, 72.5 pg / cm2. h, 33.5 pg / cm2. Logistic equation can be used to describe the relationship between cumulative secretion rate and secretion rate of hET with time of shear effect. A large number of eigenvalues and eigenvalues, which reflect the law of ET secretion, were obtained. In order to understand the pathophysiology of the in vivo vascular wall.