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利用60 Coγ射线对紫色马铃薯品种黑美人萌芽薯块进行诱变处理。诱变材料表型鉴定结果表明,辐照剂量与出苗率呈显著负相关,其半致死剂量为70Gy,致死剂量为80Gy。辐照处理能显著延缓出苗时间,导致叶片减少、茎杆变细、植株变矮。高剂量处理可提高分枝数。与CK相比,各处理结薯数差异不显著。单株产量随辐照剂量增加而降低,CK处理显著高于其他处理。在表型鉴定的基础上,利用SSR分子标记对辐射材料进行DNA检测。10对引物集团选择结果表明,6对具有多态性,共获得10条多态性条带,平均每对引物有1.7条。单株检测共获得扩增条带28条,其中8条有多态性,多态性比率为28.9%。说明,辐射可引起紫色马铃薯黑美人表型和DNA水平的变异。
Mutation treatment of purple sweet potato seed with black beauty was carried out by using 60 Coγ ray. The mutagenic material phenotype identification results showed that the radiation dose and the emergence rate was significantly negative correlation, the lethal dose of 70Gy, the lethal dose of 80Gy. Irradiation treatment can significantly delay the emergence time, leading to reduced leaves, stems thinner, shorter plants. High-dose treatment can increase the number of branches. Compared with CK, the difference between tuber number of each treatment was not significant. The yield per plant decreased with the increase of irradiation dose, CK treatment was significantly higher than other treatments. On the basis of phenotypic identification, the DNA of the irradiated materials was detected by SSR molecular markers. 10 pairs of primer group selection results showed that 6 pairs of polymorphisms, a total of 10 polymorphic bands, an average of 1.7 pairs of primers. A total of 28 bands were amplified by PCR, of which 8 were polymorphic and the percentage of polymorphic bands was 28.9%. This shows that radiation can cause variations in the phenotype and DNA level of purple black potato.