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目的 探讨雄激素阻断对膀胱癌 ＵＭ-ＵＣ-３细胞自噬与凋亡的影响作用。方法 以双氢睾酮或比卡鲁胺激活或抑制 ＵＭ-ＵＣ-３细胞 ＡＲ信号通路，以雷帕霉素或氯喹进一步诱导或抑制 ＵＭ-ＵＣ-３细胞自噬水平，根据以上不同处理条件进行相应分组。采用 Ｈｏｅｃｈｓｔ染色、流式细胞仪和 Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ检测 ＵＭ-ＵＣ-３细胞凋亡；采用透射电镜、激光共聚焦显微镜和 Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ检测 ＵＭ-ＵＣ-３细胞自噬水平。结果 雄激素去除或者 ＡＲ阻断剂比卡鲁胺可以增强膀胱癌 ＵＭ-ＵＣ-３细胞自噬和凋亡水平；雄激素阻断对 ＵＭ-ＵＣ-３细胞的凋亡促进作用可以被氯喹抑制，并且可以被雷帕霉素增强。结论 雄激素去除和 ＡＲ阻断剂比卡鲁胺可以促进 ＵＭ-ＵＣ-３细胞凋亡，这与其诱导细胞发生自噬相关。
Objective To investigate the effects of androgen on autophagy and apoptosis in bladder cancer UM-UC-3 cells. Methods Dihydrotestosterone or bicalutamide activated or inhibited the AR signaling pathway of UM-UC-3 cells and induced or inhibited the autophagy level of UM-UC-3 cells with rapamycin or chloroquine according to the above different treatment conditions Corresponding grouping. The apoptosis of UM-UC-3 cells was detected by Hoechst staining, flow cytometry and Western blotting. The autophagy of UM-UC-3 cells was detected by transmission electron microscopy, confocal laser scanning microscopy and Western blotting. Results Androgen deprivation or bicalutamide, an AR blocker, could enhance the autophagy and apoptosis of bladder cancer UM-UC-3 cells. The effect of androgen blockade on the apoptosis of UM-UC-3 cells could be inhibited by chloroquine , And can be enhanced by rapamycin. Conclusions Androgen deprivation and bicalutamide, an AR blocker, can promote the apoptosis of UM-UC-3 cells, which is related to its induction of autophagy.