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目的观察丹参对老龄小鼠肝微粒体细胞色素P450部分亚型的影响。方法给予12月龄小鼠含丹参不同剂量的药物鼠粮,连续给药三个月后,以6月龄小鼠为对照,采用荧光定量PCR法检测小鼠肝脏组织CYP1a1、CYP2a4、CYP2a5、Cyp2b9、CYP3A41、Cyp4a12、Fmo1、Fmo5等P450亚型基因m RNA的表达。结果模型组小鼠肝脏组织CYP1a1、CYP2a4、CYP2a5、Cyp2b9、CYP3a41、Cyp4a12、Fmo1、Fmo5等基因mRNA的表达量显著低于正常组小鼠;不同剂量丹参给药组肝脏CYP1a1、CYP2a4、CYP2a5、Cyp2b9、CYP3a41、Cyp4a12、Fmo1、Fmo5等基因m RNA的表达量均显著高于模型组,且丹参高剂量组作用优于低剂量组。结论衰老可导致细胞色素P450亚型基因表达减少,丹参对老龄小鼠肝微粒体CYP1a1、CYP2a4、CYP2a5、Cyp2b9、CYP3a41、Cyp4a12、Fmo1、Fmo5等基因具有诱导作用。
Objective To observe the effect of radix salviae miltiorrhizae on some subtypes of liver microsomal cytochrome P450 in aged mice. Methods 12-month-old mice were treated with different doses of Salvia miltiorrhiza. After 3 months of continuous administration, 6-month-old mice were used as control. The liver tissues of CYP1a1, CYP2a4, CYP2a5 and Cyp2b9 , CYP3A41, Cyp4a12, Fmo1, Fmo5 P450 subtype gene m RNA expression. Results The mRNA expression of CYP1a1, CYP2a4, CYP2a5, Cyp2b9, CYP3a41, Cyp4a12, Fmo1 and Fmo5 in the model group was significantly lower than that in the normal group. The liver CYP1a1, CYP2a4, CYP2a5, Cyp2b9 , CYP3a41, Cyp4a12, Fmo1, Fmo5 and other genes m RNA expression were significantly higher than the model group, and Salvia high-dose group than the low-dose group. CONCLUSION: Senescence can lead to the decrease of cytochrome P450 subtype gene expression. Salvia miltiorrhiza can induce gene expression of liver microsomal CYP1a1, CYP2a4, CYP2a5, Cyp2b9, CYP3a41, Cyp4a12, Fmo1, Fmo5 and other genes in aged mice.