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分别采用血清学方法和PCR SSP基因定型方法对 10 2份脐带血HLA A , B位点进行了检测 ,经对比分析结果显示血清学近 18.6%不能得出满意结果 ,HLA A , B位点错定率分别在6.1%和 10 .8% ,总错误率 16.9%。我们设计了 16条引物 ,以PCR SSP方法特异性扩增B15等位基因 ,并与血清学做了比较 ,发现大多数B15裂解物的血清学检测结果是错误的
Serological methods and PCR SSP gene typing method were used to detect HLA-B and HLA-B loci in 102 cord blood samples respectively. The results of comparative analysis showed that no satisfactory results were obtained with a serological grade of 18.6% Fixed rates were 6.1% and 10.8% respectively, with a total error rate of 16.9%. We designed 16 primers to specifically amplify the B15 allele using the PCR SSP method and compared it with serology and found that most of the B15 lysates had the wrong serological results