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目的:观察EPO微球剂降低小鼠免疫原性方面的优势。方法:W0W复乳法制备EPO微球,对BALB/C小鼠进行皮下注射,并利用水针剂型作为对照,采用促UT-7细胞生长抑制法检测小鼠体内的EPO抗体,观察小鼠血清中EPO抗体的量。结果:水针组小鼠血清加入细胞培养液以及一定效价的EPO溶液培养染色裂解后,吸光值比空白值有较大降低,说明其中的EPO抗体量较多。而W/O/W微球组小鼠血清中的抗体则比水针组要少,这说明EPO做成微球剂型,给药后可以减少抗体的产生,在降低免疫原性方面优于水针组。结论:微球剂型给药比水针在降低免疫原性方面具有优势。
Objective: To observe the advantages of EPO microspheres in reducing the immunogenicity of mice. Methods: The EPO microspheres were prepared by W0W double emulsion method. BALB / C mice were injected subcutaneously with water injection dosage as the control. The mouse EPO antibody was detected by UT-7 cell growth inhibition method. The amount of EPO antibody. Results: After the acupuncture mice were incubated with cell culture medium and a certain titer of EPO solution, the absorbance value of the mice was significantly lower than that of the blank value, indicating that there was more EPO antibody. However, the antibody in the serum of W / O / W microsphere group was less than that of water needle group, which indicated that the EPO was made into microsphere dosage form and could reduce antibody production after administration, which is better than water in reducing immunogenicity Needle group. CONCLUSIONS: Microsphere dosage forms have advantages over water needles in reducing immunogenicity.